PARTNERSHIPS FOR ENHANCED ENGAGEMENT IN RESEARCH (PEER) HEALTH
Development of an antigen-capture immunoassay for the rapid diagnosis of acute leptospirosis.
Summary of Recent Activities
2017 was a year of significant progress and effort in the laboratory to detect Leptospira antigen in the urine patient samples. The team has worked hard to accurately characterize and determine whether urine samples of leptospirosis patients are truly infected by Leptospira before sending the samples to Dr. Aucoin at University of Nevada Reno. They were able to succeed by developing a protocol that allowed them to detect the very low DNA Leptospira in the urine samples and now have proven laboratory SOPs to detect Leptospira in low level concentrations, both in urine and blood or even other resources samples. This has been a major breakthrough for the team as it is very tricky to detect low level concentration of Leptospira antigen, especially in urine samples.
The team is now in the process of preparing a publication entitled: Screening Urine samples using qPCR methods by SecY gene and LipL32. Dr. Ahmed from FAO/OIE and National Collaborating Centre for Reference and Research on Leptospirosis. KIT Biomedical Research in the Netherlands has agreed to review the publication draft and the team met with them during past ILS meeting in New Zealand. In addition, The team published a book, entitled 'Leptospira Detection in the Laboratory.'
Health Cycle 1 Recipients