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PARTNERSHIPS FOR ENHANCED ENGAGEMENT IN RESEARCH (PEER) HEALTH
Development of an antigen-capture immunoassay for the rapid diagnosis of acute leptospirosis.
Principal Investigator: Farida Handayani, Ministry of Health, Indonesia
NIH-Supported Collaborator: David AuCoin, University of Nevada, Reno
Title of NIH Award: Antigen Detection Assay for the Diagnosis of Melioidosis
Leptospirosis is a globally important zoonotic disease that is endemic in South East Asia with human infection commonly reported throughout the region. Leptospirosis is classified as a neglected disease in Indonesia and has been an increasing public health issue in the country. A number of outbreaks of leptospirosis have occurred in several areas in Indonesia during 2004-2012 with mortality rates as high as 35%. Laboratory diagnosis of leptospirosis is difficult due to limitations in laboratory diagnostics. Clinical diagnosis is also difficult since leptospirosis symptoms are variable, ranging from a classical flu syndrome to Weil’s disease, which typically causes major hepatic and renal failures, often leading to death. Specific laboratory tests, including bacteria isolation, DNA testing and serology using the microagglutination test (MAT), still remain limited to highly specialized laboratories.
The goal of this study is to develop a sensitive, noninvasive and inexpensive immunoassay for point-of-care diagnosis of leptospirosis.
The project will significantly improve the diagnosis of leptospirosis, as well as provide a better understanding of leptospirosis endemicity within Indonesia. This study will also aid and enhance the Ministry of Health’s efforts to control leptospirosis.
This project has three components:
1.) Production of a library of monoclonal antibodies (mAbs) specific to LPS or other circulating antigens that are secreted or shed during infection of Leptospira spp. for the development of a prototype antigen-capture ELISA.
2.) Construction of prototype immunoassay for leptospirosis diagnosis.
3.) Conduct a prevalence survey of Leptospirosis in Endemic areas in Indonesia using the immunoassay developed in Phase 2.
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